RESUMO
BACKGROUND: Wnt/ß-catenin signaling pathway is essential for the beginning of odontogenesis and may be involved in the development and progression of some odontogenic tumors. Thus, the aim of this study was to comparatively evaluate the immunohistochemical expression of Wnt/ß-catenin signaling pathway proteins in a series of AME and CCOT. MATERIAL AND METHODS: Immunohistochemical reactions were performed using antibodies against Wnt1, Wnt5a and ß-catenin in 17 cases of solid AME and 6 cases of CCOT. RESULTS: In the AME group, Wnt1 and Wnt5a were identified in the epithelium in most of the cases, and ß-catenin was mainly identified in the cytoplasm of the tumoral cells. In the CCOT group, Wnt1 and Wnt5a were identified in the epithelium and in the ghost cells in almost all the cases, and ß-catenin was mainly identified in the cytoplasm and in the nuclei of the tumoral cells. CONCLUSIONS: These results contribute to support the importance of Wnt/ß-catenin signaling pathway proteins in AME and CCOT tumorigenesis. The abnormal expression of cytoplasmic and/or nuclear ß-catenin appears to contribute to the development of both AME and CCOT. In addition, it is possible that Wnt1 and Wnt5a expression in ghost cells can contribute to its histogenesis in CCOT. Key words:Ameloblastoma, ß-catenin, calcifying cystic odontogenic tumor, immunohistochemistry, Wnt.
RESUMO
Objetivo: relatar caso clínico de tumor odontogênico adenomatoide (TOA), enfatizando suas características e diagnósticos diferenciais. Relato de caso: paciente masculino, 16 anos, apresentando lesão radiolúcida, unilocular, associada ao dente 24 incluso. Sob as hipóteses de TOA, cisto dentígero, tumor odontogênico cístico calcificante e ameloblastoma unicístico, foi realizada biópsia excisional. Na microscopia, observou-se parênquima contendo estruturas tubulares e áreas semelhantes à roseta e cribriformes, estabelecendo o diagnóstico de TOA. Conclusão: o reconhecimento das características clínicas, radiográficas e histopatológicas do TOA é essencial para guiar o processo para se estabelecer o diagnóstico definitivo e, consequentemente, permitir o tratamento adequado do paciente.
Objective: to report a case of adenomatoid odontogenic tumor (AOT), highlighting its features and differential diagnosis. Case report: a 16-year-old male with a unilocular radiolucent lesion involving the unerupted 24th tooth. Considering AOT, dentigerous cyst, calcifying cystic odontogenic tumor, and unicystic ameloblastoma as the clinical diagnoses, an excisional biopsy was performed. Microscopic analysis revealed a parenchyma containing tubular structures and roset-like areas, establishing a diagnosis of AOT. Conclusion: the acknowledgement of clinical, radiographic, and microscopic features of AOT is essential to guide a definitive diagnosis and, as a consequence, to enable a suitable treatment of the patient.
